Published
The method consists in hydrolysing with hydrochloric acid of a ground test portion, dollowed by separation of the total lysine by column chromatography and determination by spectrometry at 570 nm. Reaction of a second ground test portion with an ethanolic solution of 2,4-dinitrofluorobenzene in an alkaline medium, purification, then hydrolysis with hydrochloric acid, separation of the non-available lysine by column chromatography, and determination by spectrometry at 570 nm.
PUBLISHED
ISO 5510:1984
90.93
Standard confirmed
Dec 11, 2021
ABANDON
ISO/WD 5510
Animal feeding stuffs — Determination of available lysine
90.93 Standard confirmed