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ISO/TS 15216-2:2013

Microbiology of food and animal feed — Horizontal method for determination of hepatitis A virus and norovirus in food using real-time RT-PCR — Part 2: Method for qualitative detection

Mar 7, 2013
95.99   Withdrawal of Standard   Jul 23, 2019

General information

95.99     Jul 23, 2019

ISO

ISO/TC 34/SC 9

Technical Specification

07.100.30  

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Scope

ISO/TS 15216-2:2013 describes a method for qualitative detection of HAV and NoV genogroups I (GI) and II (GII), from test samples of foodstuffs or food surfaces. Following liberation of viruses from the test sample, viral RNA is then extracted by lysis with guanidine thiocyanate and adsorption on silica. Target sequences within the viral RNA are amplified and detected by real-time RT-PCR.
This approach is also relevant for detection of the target viruses on fomites, or of other human viruses in foodstuffs, on food surfaces or on fomites following appropriate validation and using target-specific primer and probe sets.

Life cycle

NOW

WITHDRAWN
ISO/TS 15216-2:2013
95.99 Withdrawal of Standard
Jul 23, 2019

REVISED BY

PUBLISHED
ISO 15216-2:2019

National adoptions

SRPS CEN ISO/TS 15216-2:2014  
ISS

Microbiology of food and animal feed - Horizontal method for determination of hepatitis A virus and norovirus in food using real-time RT-PCR - Part 2: Method for qualitative detection (ISO/TS 15216-2:2013, Corrected Version 2013-05-01)

95.99   Withdrawal of Standard

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