ISO/CD 21686-2

Water quality — In vivo fluorescence measurement of the endocrine disrupting effects of natural water and wastewater using transgenic embryos — Part 2: Estrogen Activity (Oryzias latipes)

Scope

This document specifies a method for the determination of the estrogen activity of water and waste water by means of a reporter gene assay with genetically modified Oryzias latipes (O. latipes, medaka) eleutheroembryos.

This method is applicable to:
- fresh water;
- waste water;
- aqueous extracts and leachates;
- eluates of sediments (fresh water);
- pore water;
- aqueous solutions of single substances or of chemical mixtures;
- drinking water.

NOTE 1: Extraction, pre-concentration or dilution of water samples can prove necessary

The organism preparation methods (freeze-drying, extraction, mineralization) and quantification of the chemical substances do not fall within the scope of this document.