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ISO/DIS 25300

Soil conditioners and beneficial substances — Determination of free amino acids

General information

40.60     Jan 7, 2026

ISO

ISO/TC 134

International Standard

65.080  

Scope

This document specifies the methods for determining the content of free amino acids in fertilizers, including the use of automatic amino acid analyzers, pre-column derivatization followed by high-performance liquid chromatography and high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS). These methods are applicable to the following amino acids: Asparagine Tryptophan, Glutamine, Theanine, Aspartic acid, Threonine, Serine, Glutamic acid, Proline, Glycine, Alanine, Valine, Methionine, Isoleucine, Leucine, Tyrosine, Phenylalanine, Histidine, Lysine, Arginine, and Cysteine.

Life cycle

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PROJECT
ISO/DIS 25300
40.60 Close of voting
Jan 7, 2026