ISO/CD 21160-2

Cigarettes — Determination of selected carbonyls in the mainstream smoke with a smoking regime according to ISO 3308 (standard smoking regime) using reversed phase high performance liquid chromatography with ultra-violet/diode array detection (HPLC-UV/DAD) — Part 2: Method using impregnated glass fibre filter pads

Scope

This document specifies a method for the determination of selected carbonyls (formaldehyde, acetaldehyde, acetone, acrolein, propionaldehyde, crotonaldehyde, 2-butanone and butyraldehyde) as their 2,4-dinitrophenylhydrazones in mainstream smoke using reversed phase HPLC-UV/DAD with impregnated glass fibre filter pad.
This method is applicable to cigarettes with nicotine-free dry particulate matter (NFDPM) yields between 1 mg/cigarette and 15 mg/cigarette using reversed phase HPLC-UV/DAD.
Note: The analytical method cannot separate n-butyraldehyde and iso-butyraldehyde and therefore the method reports butyraldehyde (sum of n-butyraldehyde and iso-butyraldehyde) based on the response factor of n-butyraldehyde.