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SRPS ISO 4052:1997

Coffee — Determination of caffeine content (Reference method)

Jun 26, 1997
Jun 26, 1997
95.99   Withdrawal of Standard   Dec 23, 2014

General information

95.99     Dec 23, 2014

ISS

E034-7,8,15,18

International Standard

67.140.20  

Serbian  

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Scope

The method consists in extracting of the caffeine from a test portion, in an ammoniacal medium, successive purifying, with diethyl ether, on two chromatographic columns, the first in an alkaline medium, the second in an acid medium, followed by elution by chloroform. Spectrometric measurement of the eluate at the wavelength of maximum absorbance (in the ultraviolet region). A first figure shows examples of chromatographic columns. A second figure gives an example of spectrometric measurement.

Life cycle

NOW

WITHDRAWN
SRPS ISO 4052:1997
95.99 Withdrawal of Standard
Dec 23, 2014

Related project

Adopted from ISO 4052:1983