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ISO 11063:2020

Soil quality — Direct extraction of soil DNA

Sep 29, 2020

General information

90.20     Jul 15, 2025

ISO

ISO/TC 190/SC 4

International Standard

13.080.30  

English   French  

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Scope

The present document specifies a method for direct extraction of DNA from soil samples to analyse the abundance and composition of microbial communities by various techniques of molecular biology including real-time quantitative PCR (qPCR). This method is mainly dedicated to agricultural and forest soils. This method can possibly not be suitable for soils rich in organic matter (e.g. peat soils) or soils heavily polluted with organic pollutants or heavy metals.
The direct extraction of DNA from soil samples provides unique insight into the α- and β-diversity of microbial communities. Next-generation sequencing of amplicons obtained by PCR (polymerase chain reaction) amplification of soil DNA constitutes a promising domain which will in the near future contribute to the development of routine tools to monitor microbial communities in soil environments.

Life cycle

PREVIOUSLY

WITHDRAWN
ISO 11063:2012

NOW

PUBLISHED
ISO 11063:2020
90.20 Standard under periodical review
Jul 15, 2025

National adoptions

delSRPS EN ISO 11063 rev:2018  
ISS

Soil quality - Direct extraction of soil DNA (ISO 11063:2020)

10.98   New project rejected

H190 more

SRPS EN ISO 11063:2021  
ISS

Soil quality - Direct extraction of soil DNA (ISO 11063:2020)

60.60   Standard published

Z183 more

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