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Glavni meni

ISO 11063:2020

Soil quality — Direct extraction of soil DNA
29. 9. 2020.

Опште информације

90.20     15. 7. 2025.

ISO

ISO/TC 190/SC 4

Međunarodni standard

13.080.30  

engleski   francuski  

Kupovina

Objavljen

Jezik na kome želite da primite dokument.

Apstrakt

The present document specifies a method for direct extraction of DNA from soil samples to analyse the abundance and composition of microbial communities by various techniques of molecular biology including real-time quantitative PCR (qPCR). This method is mainly dedicated to agricultural and forest soils. This method can possibly not be suitable for soils rich in organic matter (e.g. peat soils) or soils heavily polluted with organic pollutants or heavy metals.
The direct extraction of DNA from soil samples provides unique insight into the α- and β-diversity of microbial communities. Next-generation sequencing of amplicons obtained by PCR (polymerase chain reaction) amplification of soil DNA constitutes a promising domain which will in the near future contribute to the development of routine tools to monitor microbial communities in soil environments.

Životni ciklus

PRETHODNO

POVUČEN
ISO 11063:2012

TRENUTNO

OBJAVLJEN
ISO 11063:2020
90.20 Početak postupka preispitivanja standarda
15. 7. 2025.

Nacionalna preuzimanja

Kvalitet zemljišta — Metoda direktne ekstrakcije DNK iz uzoraka zemljišta

10.98   Novi projekat se odbacuje

Kvalitet zemljišta — Metoda direktne ekstrakcije DNK iz uzoraka zemljišta

60.60   Standard objavljen

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