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ISO/TS 4985:2023

Milk and milk products — Determination of alkaline phosphatase activity — Fluorimetric microplate method

Jul 31, 2023

General information

90.20     Jul 15, 2026

ISO

ISO/TC 34/SC 5

Technical Specification

67.100.10  

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This document specifies a fluorimetric microplate method for the determination of alkaline phosphatase (ALP, EC 3.1.3.1)[5] activity in raw and heat-treated whole milk, semi-skimmed milk, skimmed milk, cream, flavoured milks and cheeses.
This method is applicable to milk and milk-based drinks from cows, sheep and goats. Although the method was not tested in milk from other species, it can also be applicable to milk from other species with a similar composition to cow, sheep or goat milk, such as milk from buffalo and camelids. It is also applicable to milk powder after reconstitution and soft, semi-hard and hard cheeses provided that the mould is only on the surface of the cheese and not also in the inner part (e.g. blue veined cheeses). For large hard cheeses, specific conditions of sampling apply (see Clause 7).
NOTE            This method was adapted from Reference [6].

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PUBLISHED
ISO/TS 4985:2023
90.20 Standard under periodical review
Jul 15, 2026

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