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SRPS ISO 4133:2002

Meat and meat products — Determination of glucono-delta-lactone content (Reference method)

Nov 28, 2002
Nov 28, 2002
95.99   Withdrawal of Standard   Jan 28, 2013

General information

95.99     Jan 28, 2013

ISS

E034-6

International Standard

67.120.10  

Serbian  

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Scope

The method consists in extracting of the compound with ice-cold perchloric acid solution, centrifuging, decanting and filtrating, followed by hydrolysis of the compound in a portion of the filtrate with potassium hydroxide into gluconate. Transformation of the gluconate in the extract by the following reactions: 1) with adenosine-5-triphosphate (ATP) and 2) with concomitant reduction of an equivalent amount of nicotinamide adenine dinucleotide phosphate (NADP). Photometric measurement of the amount of nicotinamide adenine dinucleotide phosphate (reduced) (NADPH) formed.

Life cycle

NOW

WITHDRAWN
SRPS ISO 4133:2002
95.99 Withdrawal of Standard
Jan 28, 2013

Related project

Adopted from ISO 4133:1979