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Glavni meni

ISO/TS 13136:2012

Microbiology of food and animal feed — Real-time polymerase chain reaction (PCR)-based method for the detection of food-borne pathogens — Horizontal method for the detection of Shiga toxin-producing Escherichia coli (STEC) and the determination of O157, O111, O26, O103 and O145 serogroups
7. 11. 2012.

Опште информације

90.92     12. 7. 2022.

ISO

ISO/TC 34/SC 9

Tehnička specifikacija

07.100.30  

engleski   francuski  

Kupovina

Objavljen

Jezik na kome želite da primite dokument.

Apstrakt

ISO/TR 13136:2012 describes the identification of Shiga toxin-producing Escherichia coli (STEC) by means of the detection of the following genes: a) the major virulence genes of STEC, stx and eae; b) the genes associated with the serogroups O157, O111, O26, O103, and O145.
In any case, when one or both of the stx genes is/are detected, the isolation of the strain is attempted.
The isolation of STEC from samples positive for the presence of the genes specifying the serogroups in the scope of this method can be facilitated by using serogroup-specific enrichment techniques (e.g. immunomagnetic separation, IMS).
The protocol uses real-time PCR as the reference technology for detection of the virulence and serogroup-associated genes.
ISO/TR 13136:2012 is applicable to: 1) products intended for human consumption and the feeding of animals; 2) environmental samples in the area of food production and food handling; 3) environmental samples in the area of primary production.

Životni ciklus

TRENUTNO

OBJAVLJEN
ISO/TS 13136:2012
90.92 Odluka o izmeni ili reviziji standarda
12. 7. 2022.

REVIDIRAN OD

PROJEKAT
ISO/DIS 13136-1

PROJEKAT
ISO/DIS 13136-2

Nacionalna preuzimanja

Mikrobiologija hrane i hrane za životinje — Metoda koja se zasniva na lančanoj reakciji polimeraze (PCR) u realnom vremenu za otkrivanje patogenih mikroorganizama u hrani — Horizontalna metoda za otkrivanje Escherichia coli koja proizvodi šiga toksin (STEC) i određivanje serogrupa O157, O111, O26, O103 i O145

60.60   Standard objavljen

E034-9 Saznaj više