ISO 17601:2016

Soil quality — Estimation of abundance of selected microbial gene sequences by quantitative PCR from DNA directly extracted from soil

Datum objavljivanja: 12. 1. 2016.

Опште информације

Trenutna faza: 90.92 Effective date: 24. 10. 2023.

Inicijator: ISO

Pripada: ISO/TC 190/SC 4

Vrsta: Međunarodni standard

ICS: 13.080.30

Jezik: engleski francuski

Kupovina

Status: Objavljen

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Apstrakt

ISO 17601:2016 specifies the crucial steps of a quantitative real-time polymerase chain reaction (qPCR) method to measure the abundance of selected microbial gene sequences from soil DNA extract which provides an estimation of selected microbial groups.
It is noteworthy that the number of genes is not necessarily directly linked to the number of organisms that are measured. For example, the number of ribosomal operon is ranging from one copy to 20 copies in different bacterial phyla. Therefore, the number of 16S rRNA sequences quantified from soil DNA extracts does not give an exact estimate of the number of soil bacteria. Furthermore, the number of sequences is not necessarily linked to living microorganisms and can comprise sequences amplified from dead microorganisms.

Životni ciklus

TRENUTNO

OBJAVLJEN
ISO 17601:2016
90.92 Odluka o izmeni ili reviziji standarda
24. 10. 2023.

REVIDIRAN OD

PROJEKAT
ISO/FDIS 17601

Nacionalna preuzimanja

SRPS EN ISO 17601:2018

ISS

Kvalitet zemljišta – Procena zastupljenosti izabranih sekvenci mikrobioloških gena kvantitativnim PCR iz DNK koji je direktno ekstrahovan iz zemljišta

60.60 Standard objavljen

H190 Saznaj više