ISO/DIS 21569-1

Horizontal methods for molecular biomarker analysis — Methods of analysis for the detection of genetically modified organisms and derived products — Part 1: Qualitative nucleic acid based methods

Apstrakt

This International Standard describes the procedure to qualitatively detect genetically modified organisms
(GMOs) and derived products by analysing the nucleic acids extracted from the sample under study. The main
focus is on polymerase chain reaction (PCR) based amplification methods.
It gives general requirements for the specific detection and identification of target nucleic acid sequences
(DNA) and for the confirmation of the identity of the amplified DNA sequence.
Guidelines, minimum requirements and performance criteria laid down in this International Standard are
intended to ensure that comparable, accurate and reproducible results are obtained in different laboratories.
This International Standard has been established for food matrices, but could also be applied to other
matrices (e.g. feed and plant samples from the environment).
Specific examples of methods are provided in Annexes A to D.

Nacionalna preuzimanja